Paternity testing using 21 STR Loci in a biotechnology approach: case of Rwandan Population

INTRODUCTION: We focused on a sample size of 141 unrelated Rwandan persons to genotype21 STR loci that were relied up in establishing allele frequencies, heterozygosity and power ofexclusion. This study aims at exploring frequencies representative from Rwandanpopulation determine probability paternity for sampled families basing polymorphic STRsloci, using 21 autosomal-STR by Genetic Analyzer 3500X.METHODS: was an experimental global filer TM Express PCR Amplification kit wasused amplify autosomal loci.RESULTS: The total number observed alleles 270; the largest different alleleswas seen SE33 D18S51 loci. locus with highest SE33, while locusTH01 had lowest heterozygosity. ranged 71.3%(TH01) 91.6% (SE33) average 81.1% good indicator high genetic variability. Forall microsatellites analyzed exclusion 43.4% (TH01) 78.1% (SE33)with 58.2%. For seven eight cases examined test alleged father wasnot excluded as biological child. results found examination case 8 indicated thatthe not child.CONCLUSION: Based calculated statistical parameters, population Rwanda may usethese vital tool forensic identification testing.